Help please for AP BIO Transformation Lab and DNA fingerprinting Lab?
Wednesday, November 4th, 2009 at
8:15 pm
Please help me answer these questions:
1) What other methods can be used to verify that transformation occurred? Explain.
2)In your lab, you ran your DNA samples on a 0.8% agarose gel. Would you get the same results if you ran your samples on a higher percentage agarose gel? Why or why not?
3) If you have a restriction enzyme that cuts a piece of DNA at two recognition sites, how many DNA fragments would you see on a gel?
Thank you=]
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Tagged with: dna fragments • dna samples • restriction enzyme • transformation
Filed under: DNA Fingerprinting
1. Grow on selective or differential media.
2. The same number of bands but the migration pattern would be different due the the different migration of the DNA fragments based on length. Shorter DNA-higher percentage gel is appropriate, longer DNA- lower percentage gel is appropriate.
3. Three, assuming all the fragments are not so small that they run off the gel.
Were you not paying attention in class?