Why treat plasmid DNA with alkaline phosphatase when making a recombinant plasmid?

Sunday, November 1st, 2009 at 1:52 am  
Comments (2)

When attempting to create a recombinant DNA molecule, by inserting a foreign sequence into the vector plasmid; what is the point (and how does it achieve it) of treating the plasmid DNA with alkaline phosphatase?

I think it has something to do with the orientation of the inserted sequence, but unsure how the treatment achieves this.
thats right, it makes sense now
thanks guys

In DNA profiling, what is the function of alkaline phosphatase ? ?

Friday, October 30th, 2009 at 7:28 pm  
Comments (2)

In DNA profiling, what is the function of alkaline phosphatase? ...

Please keep your answer clear, do not make it complicate, just explain the answer by your own!...thank you!