Please help me answer these questions:

1) What other methods can be used to verify that transformation occurred? Explain.

2)In your lab, you ran your DNA samples on a 0.8% agarose gel. Would you get the same results if you ran your samples on a higher percentage agarose gel? Why or why not?

3) If you have a restriction enzyme that cuts a piece of DNA at two recognition sites, how many DNA fragments would you see on a gel?

Thank you=]

1 Restriction enzymes cut apart random sequences of DNA.

2 "Cutting" by restriction enzymes results in DNA fragments with sticky ends.

3 DNA ligase forms weak bonds between the different DNA fragments.

4 The 2 DNA fragments from the original restriction site recombine together again, making recombinant DNA.

a. involves inserting normal genes into cells with a genetic disorder
b. involves a vector, and DNA sequences does not
c. compares DNA fragments rather than the nucleotide order of DNA
d. makes millions of copies of DNA pieces

Please choose for th best answer and explain it..
tnx!