Describe a recombinant DNA molecule and explain how to make and screen for recombinants using restriction enzymes, gel electrophoresis, ligase, vector and transformation.
Help? I can't seem to find any notes on this online.)=
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When attempting to create a recombinant DNA molecule, by inserting a foreign sequence into the vector plasmid; what is the point (and how does it achieve it) of treating the plasmid DNA with alkaline phosphatase?
I think it has something to do with the orientation of the inserted sequence, but unsure how the treatment achieves this.
thats right, it makes sense now
thanks guys
Is a transgenic hybrid a DNA molecule consisting of 2 or more DNA segments that are NOT found together in nature? Or is it recombinant DNA?
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