what's the benefit of using bacterial cells for recombinant DNA?

I'm doing a Recombinant DNA "paper" plasmids lab.

When attempting to create a recombinant DNA molecule, by inserting a foreign sequence into the vector plasmid; what is the point (and how does it achieve it) of treating the plasmid DNA with alkaline phosphatase?

I think it has something to do with the orientation of the inserted sequence, but unsure how the treatment achieves this.
thats right, it makes sense now
thanks guys

how can recombinant DNA be used to insert a gene of interest into a bacterium?

1 Restriction enzymes cut apart random sequences of DNA.

2 "Cutting" by restriction enzymes results in DNA fragments with sticky ends.

3 DNA ligase forms weak bonds between the different DNA fragments.

4 The 2 DNA fragments from the original restriction site recombine together again, making recombinant DNA.

As the title asks: What are two ways in which people benefit from recombinant DNA?

Interferon and insulin have been produced by _______.
What are the methods for producing recombinant DNA referred to as?

Thanks a lot!

<3 Musikk

I have a bio paper due soon and I can't figure out all of the methods. My teacher wanted the four major methods of genetic engineering and so far I've narrowed it down to the vector method, bioballistics, the plasmid method and recombinant DNA. The plasmid method and recombinant DNA sound nearly identical and if they are the same then what would the fourth method be?

Is a transgenic hybrid a DNA molecule consisting of 2 or more DNA segments that are NOT found together in nature? Or is it recombinant DNA?

Recombinant DNA:
A. contains DNA from only one organism
B. is composed of RNA
C. contains DNA from two or more different sources
D. does not exist in nature

Recombinant DNA:
A. contains DNA from only one organism
B. is composed of RNA
C. contains DNA from two or more different sources
D. does not exist in nature