a. restriction enzyme
b. reverse transcriptase
c. ligase
d. DNA polymerase
e. RNA polymerase

Please help me answer these questions:

1) What other methods can be used to verify that transformation occurred? Explain.

2)In your lab, you ran your DNA samples on a 0.8% agarose gel. Would you get the same results if you ran your samples on a higher percentage agarose gel? Why or why not?

3) If you have a restriction enzyme that cuts a piece of DNA at two recognition sites, how many DNA fragments would you see on a gel?

Thank you=]

I have this lab in which I have to Identify DNA by Restriction Enzyme Pattern.Why more than one Enzyme is used for DNA fingerprinting? Could DNA samples have been distinguished from one another if only one Enzyme is used? How to analyze gel Band patterns if two enzymes are used?