Describe a recombinant DNA molecule and explain how to make and screen for recombinants using restriction enzymes, gel electrophoresis, ligase, vector and transformation.
Help? I can't seem to find any notes on this online.)=
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1 Restriction enzymes cut apart random sequences of DNA.
2 "Cutting" by restriction enzymes results in DNA fragments with sticky ends.
3 DNA ligase forms weak bonds between the different DNA fragments.
4 The 2 DNA fragments from the original restriction site recombine together again, making recombinant DNA.
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