Why treat plasmid DNA with alkaline phosphatase when making a recombinant plasmid?
Sunday, November 1st, 2009 at
1:52 am
Comments (2)
When attempting to create a recombinant DNA molecule, by inserting a foreign sequence into the vector plasmid; what is the point (and how does it achieve it) of treating the plasmid DNA with alkaline phosphatase?
I think it has something to do with the orientation of the inserted sequence, but unsure how the treatment achieves this.
thats right, it makes sense now
thanks guys
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