Describe a recombinant DNA molecule and explain how to make and screen for recombinants using restriction enzymes, gel electrophoresis, ligase, vector and transformation.
Help? I can’t seem to find any notes on this online.)=
In recombinant DNA technology, what is the object that is used to carry new DNA into a cell?
A. Restriction enzyme
B. Carrier
C. Vector
D. Nuclear transfer
When attempting to create a recombinant DNA molecule, by inserting a foreign sequence into the vector plasmid; what is the point (and how does it achieve it) of treating the plasmid DNA with alkaline phosphatase?
I think it has something to do with the orientation of the inserted sequence, but unsure how the treatment achieves this.
thats [...]
I have a bio paper due soon and I can’t figure out all of the methods. My teacher wanted the four major methods of genetic engineering and so far I’ve narrowed it down to the vector method, bioballistics, the plasmid method and recombinant DNA. The plasmid method and recombinant DNA sound nearly identical [...]
a. involves inserting normal genes into cells with a genetic disorder
b. involves a vector, and DNA sequences does not
c. compares DNA fragments rather than the nucleotide order of DNA
d. makes millions of copies of DNA pieces
Please choose for th best answer and explain it..
tnx!
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